Why is it essential to include both dead and live tissue in pathogen cultures?

Including both dead and live tissue in pathogen cultures is essential because pathogens can survive in various forms, including both living and dead host materials. This is particularly important for understanding the dynamics of infection and survival of plant pathogens.

Live tissue provides a habitat where pathogens can actively grow and reproduce, allowing researchers to observe their effects on living plants and understand their pathogenicity. On the other hand, dead tissue can harbor viable pathogens that remain capable of causing disease under the right conditions, even after the plant has died. By including samples from both types of tissue, researchers can obtain a more comprehensive view of the pathogen's lifecycle, survival strategies, and potential threat to healthy plants. This approach enhances the accuracy and reliability of diagnostic and research outcomes, leading to better management and control strategies for plant diseases.