How does the inclusion of live tissue in samples assist in identifying pathogens?

The inclusion of live tissue in samples is essential for identifying pathogens because live tissue can host active pathogens, allowing for the observation of the pathogen's behavior and infection characteristics. When examining live tissue, pathologists can see how the pathogen interacts with the plant, which can provide critical insights into its life cycle, virulence, and reproductive mechanisms. This dynamic interaction cannot be observed in dead tissue, where the pathogen may have already ceased activity or undergone decay, limiting identification capabilities.

Identifying pathogens in live samples also enhances the chances of detecting specific symptoms associated with the pathogen's activity, such as tissue discoloration, wilting, or lesions. These symptoms are fundamental for diagnosis and can guide more effective management strategies. In contrast, relying solely on dead tissue might not reveal the active presence or resilience of a pathogen, as many pathogens can survive in the environment without causing immediate visible damage.

Thus, utilizing live tissue is a crucial tactic in plant pathology, improving the accuracy and efficiency of pathogen identification.